Vozes do Sul Global: a midiatização dos feminismos em um podcast de mulheres brasileiras pelo mundo / Voces del Sur Global: la mediatización de los feminismos en un podcast de mujeres brasileñas por el mundo / Voices from the Global South: the mediatization of feminisms in a podcast by Brazilian women around the world

Resumo O podcast Femigrantes BR se define como "um espaço de conversa de mulheres feministas e imigrantes pelo mundo". Em maio de 2022, o Femigrantes BR contava com 21 episódios, reunindo histórias de brasileiras que vivem e atuam em diversos países. Na voz das entrevistadas, histórias de vida expandem-se para além do relato pessoal, rico em si mesmo, para conectar-se com estruturas solidamente assentadas como as do racismo e das relações desiguais entre Norte e Sul Global. A partir do objeto e do cenário descritos, procuro investigar qual tem sido a contribuição dos feminismos, enquanto discurso político, para o "empoderamento" dessas mulheres migrantes e analisar como a midiatização do debate feminista reflete na forma como estas atuam na vida pública do país no qual passaram a viver. No esforço para atender a esses pontos, dedico-me a um trabalho imersivo nos meios digitais e, em diálogo com aportes dos estudos feministas da tecnologia, decoloniais, pós-coloniais e da comunicação midiática, intento pensar como ocupação feminista da internet pode potencializar transformações sociais nas estruturas desiguais de gênero e contribuir para desafiar as hierarquias entre Norte e Sul Global.

Resumen El podcast Femigrantes BR se define como "un espacio de conversación de mujeres feministas e inmigrantes por el mundo". En mayo de 2022, Femigrantes BR tuvo 21 episodios, que reunieron historias de mujeres brasileñas que viven y trabajan en varios países. En las voces de las entrevistadas, las historias de vida se expanden más allá del relato personal, rico en sí mismo, para conectarse con estructuras firmemente establecidas como las del racismo y de las relaciones desiguales entre el Norte y el Sur Global. A partir del objeto y el escenario descritos, busco investigar cuál ha sido la contribución de los feminismos, como discurso político, al empoderamiento de estas mujeres migrantes y analizar cómo la cobertura mediática del debate feminista se refleja en la forma de actuar en la vida pública del país en el que ahora viven. En el esfuerzo por atender estos puntos, me dedico a un trabajo de inmersión en los medios digitales y, en diálogo con aportes de los estudios feministas de la tecnología, decoloniales, poscoloniales y de la comunicación mediática, busco pensar cómo la ocupación feminista de la internet puede potenciar transformaciones sociales en las estructuras desiguales de género y contribuir a desafiar las jerarquías entre el Norte y el Sur Global.

Abstract The podcast Femigrantes BR is defined as "a space for conversation among feminist-immigrant women around the world". The podcast had 21 episodes, gathering stories of Brazilian women who live and work in several countries. In the voices of the interviewees, life stories expand beyond the personal account and connect with firmly established structures such as racism and the unequal relations between the Global North and South. Taking that object and scenario, I investigate what has been the contribution of feminisms, as political discourse, to the "empowerment" of those migrant women and analyse how the media coverage of the feminist debate reflects on the way they act in the public life of the country in which they now live. In the effort to attend to these points, I engage in an immersion in digital media and, in dialogue with contributions from feminist media, technology, decolonial, postcolonial studies, I discuss how the feminist occupation of the internet can potentiate social transformations in unequal gender structures and contribute to challenge hierarchies between North and Global South.

Effects of Costunolide on Cell Cycle Distribution and Apoptosis of Breast Cancer SK-BR- 3 Cells / 中国药房

OBJECTIVE：To study the effects of costunolide （COS）on the cell cycle distribution and apoptosis of human breast cancer SK-BR- 3 cells and its mechanism. METHODS ：Human breast cancer SK-BR- 3 cells were divided into blank control group，and COS groups of 10，20，30，40，50 μmol/L. MTT assay was used to detect the effects of COS on cell proliferation. SK-BR-3 cells were divided into blank control group ，COS low ，medium and high concentration groups （10，20，30 μmol/L）. After cultured for 24 h，flow cytometry was used to detect the distribution of cell cycle. Hoechst 33258 fluorescence staining was used to detect cell apoptosis. Western blot assay was used to detect the expression of p 53，caspase-3，Bcl-2，Bax，p21，CDK2 and cyclinE. RESULTS ：Compared with blank control group ，COS could significantly inhibit the proliferation of SK-BR- 3 cells（P＜ 0.05 or P＜0.01），and in a dose and time-dependent manner. Low ，medium and high concentrations of COS could induce cell apoptosis and arrest cell at G 1/S phase （P＜0.05 or P＜0.01），could significantly up-regulate the protein expression of p 53， caspase-3，Bax and p 21（P＜0.05 or P＜0.01），and could significantly down-regulate the protein expression of Bcl- 2，CDK2 and cyclinE（P＜0.01）. CONCLUSIONS ：COS can inhibit the proliferation of human breast cancer SK-BR- 3 cells and induce cell apoptosis and cell cycle arrest. The mechanism may be related to the regulation of p 53/Bax/Bcl-2/caspase-3 apoptosis signal pathway.

Escape from abluminal LRP1-mediated clearance for boosted nanoparticle brain delivery and brain metastasis treatment

Breast cancer brain metastases (BCBMs) are one of the most difficult malignancies to treat due to the intracranial location and multifocal growth. Chemotherapy and molecular targeted therapy are extremely ineffective for BCBMs due to the inept brain accumulation because of the formidable blood‒brain barrier (BBB). Accumulation studies prove that low density lipoprotein receptor-related protein 1 (LRP1) is promising target for BBB transcytosis. However, as the primary clearance receptor for amyloid beta and tissue plasminogen activator, LRP1 at abluminal side of BBB can clear LRP1-targeting therapeutics. Matrix metalloproteinase-1 (MMP1) is highly enriched in metastatic niche to promote growth of BCBMs. Herein, it is reported that nanoparticles (NPs-K-s-A) tethered with MMP1-sensitive fusion peptide containing HER2-targeting K and LRP1-targeting angiopep-2 (A), can surmount the BBB and escape LRP1-mediated clearance in metastatic niche. NPs-K-s-A revealed infinitely superior brain accumulation to angiopep-2-decorated NPs-A in BCBMs bearing mice, while comparable brain accumulation in normal mice. The delivered doxorubicin and lapatinib synergistically inhibit BCBMs growth and prolongs survival of mice bearing BCBMs. Due to the efficient BBB penetration, special and remarkable clearance escape, and facilitated therapeutic outcome, the fusion peptide-based drug delivery strategy may serve as a potential approach for clinical management of BCBMs.

Effect of down-regulation of lncRNA LINC00263 targeting miR-4458 on regulating radiosensitivity of breast cancer SK-BR-3 cells / 中华放射肿瘤学杂志

Objective:To evaluate the effect of down-regulating lncRNA LINC00263 targeting miR-4458 on the proliferation, migration, invasion and radiosensitivity of breast cancer SK-BR-3 cells.Methods:The expression differences of LINC00263 in breast cancer tissues, adjacent tissues, normal breast epithelial cells and breast cancer cells were determined by qRT-PCR. Transfection of LINC00263 shRNA in breast cancer SK-BR-3 cells down-regulated the expression of LINC00263, and the cloning experiment was used to detect the radiosensitivity. Breast cancer SK-BR-3 cells were treated with 6 Gy irradiation. CCK-8 assay was employed to detect cell proliferation. Flow cytometry was adopted to detect cell apoptosis. Transwell chamber test was performed to detect cell migration and invasion. Western blot was used to detect the expression levels of C-Caspase-3 and C-Caspase-9, MMP-2 and MMP-9 proteins. Bioinformatics software predicted that LINC00263 and miR-4458 had complementary binding sites, and the luciferase reporter system was utilized determine the targeting relationship between LINC00263 and miR-4458. LINC00263 shRNA and miR-4458 inhibitor were co-transfected into breast cancer SK-BR-3 cells, and 6 Gy irradiation was given to detect the changes in cell proliferation, apoptosis, invasion and migration.Results:The expression level of LINC00263 in breast cancer tissues was higher than that in adjacent tissues. The expression level of LINC00263 in breast cancer cells was higher compared with that in normal breast epithelial cells. The radiosensitivity of breast cancer SK-BR-3 cells was increased after transfection of LINC00263 shRNA. Transfection of LINC00263 shRNA and radiation exerted a synergistic effect, jointly inhibited breast cancer cell proliferation, migration and invasion, promoted cell apoptosis, up-regulated the expression levels of C-Caspase-3 and C-Caspase-9 proteins in cells, and down-regulated those of MMP-2 and MMP-9 proteins. Down-regulation of LINC00263 targetedly up-regulated miR-4458 expression. miR-4458 inhibitor reversed the inhibitory effect of LINC00263 shRNA combined with radiation on the proliferation, migration, invasion and apoptosis promotion of breast cancer SK-BR-3 cells.Conclusion:Down-regulating lncRNA LINC00263 targeting miR-4458 inhibits the proliferation, migration and invasion of breast cancer SK-BR-3 cells, and improves cell radiosensitivity.

Consumption of dry matter observed and predicted by the nutritional systems in senepol bulls kept in confinement / Consumo de matéria seca observado e previsto pelos sistemas nutricionais em touros senepol mantidos em confinamento

The objective was to compare the dry matter consumption (CMS) observed, through the use of the GrowSafe® electronic system, with that predicted by the BR-Corte (2010 and 2016) and NRC (2000) nutritional systems in confined Senepol bulls. To this end, 24 Senepol Bulls were used in a completely randomized design, uncastrated with an average initial weight of 368 kg and 16 months of age. The evaluation of the accuracy and approximation of the CMS estimates by the nutritional systems was adjusted by the simple linear regression model and the decomposition of the mean square of the prediction error (QMEP). The mean CMS observed was 10.33 kg.day-1, higher than the values predicted by the nutritional systems, in which the values predicted by the NRC (2000) and BR-Corte 2010 and 2016 underestimated the CMS by 29.62, 6.19 and 2.03%, respectively. The verification of QMEP and its decomposition made it possible to infer the proximity of the values predicted by the BR-Corte 2010 and 2016 models and the values observed, which presented a better adjustment in relation to the NRC. Surprisingly the values predicted by the NRC, created from a database with Bos taurus animals, showed greater distance from the values predicted and observed, and it was expected greater accuracy of the NRC models for this category and animal breed. It is concluded that the BR-Corte 2016 was the most appropriate model to estimate the CMS of confined Senepol bulls.

Objetivou-se comparar o consumo de matéria seca (CMS) observado, por meio do uso do sistema eletrônico GrowSafe®, com o predito pelos sistemas nutricionais BR-Corte (2010 e 2016) e NRC (2000) em touros da raça Senepol em confinamento. Para isso, foram utilizados 24 Touros da raça Senepol em delineamento inteiramente casualizado, não castrados com peso inicial médio 368 kg e 16 meses de idade. A avaliação da exatidão e aproximação das estimativas do CMS pelos sistemas nutricionais foi ajustada pelo modelo de regressão linear simples e decomposição do quadrado médio do erro de predição (QMEP). O CMS médio observado foi de 10,33 kg.dia-1, valor superior aos valores preditos pelos sistemas nutricionais, no qual os valores preditos pelo NRC (2000) e BR-Corte 2010 e 2016 subestimaram o CMS em 29,62, 6,19 e 2,03%, respectivamente. A verificação do QMEP e sua decomposição possibilitou inferir sobre a proximidade dos valores preditos pelos modelos BR-Corte 2010 e 2016 e os valores observados, os quais apresentaram melhor ajuste em relação ao NRC. Surpreendentemente os valores preditos pelo NRC, criado a partir de banco de dados com animais Bos taurus, apresentou maior distanciamento dos valores preditos e observados, sendo que era esperada maior precisão dos modelos NRC para esta categoria e raça animal. Conclui-se que o BR-Corte 2016 foi o modelo mais adequado para estimar o CMS de touros da raça Senepol em confinamento.

Assessment of Cytokeratin Expression in Carcinoma Breast

Breast cancer is the most common malignancy in females and an important cause of cancer deaths worldwide. With advances in oncopathology, breast cancers are now diagnosed and treated at a molecular level. To distinguish and subdivide the type of cancer with the suitable markers and to foresee its prognosis and therapeutic approach, cytokeratin can be used which is prognostic tumour marker and has a number of different advantages as it is an important IHC marker, cytokeratin (Ck) is epithelial intermediate filament, which is expressed in a tissue specific manner. Expression of the intermediate filaments, predominantly cytokeratins (Ck) reflects the epithelial cell type. In breast, the luminal epithelial cells express CK 8 / 18, CK 7 and CK 19, while basal cell expresses CK 5 / 6, CK 14 and CK 17. Bloom Richardson grading (BR) system has a powerful prognostic value. It is also incorporated in the algorithm for the determination in the use of adjuvant chemotherapy. Moreover, as a tumour marker cytokeratin can accurately predict disease status as it is easy, inexpensive and a reliable tool for efficient management. CK and BR grading can be used as cost effective diagnostic tools in hormone positive breast carcinoma for diagnosis as well as treatment. Very early detection of breast cancer reduces the mortality and morbidity.

Chemical constituents of triterpenes from Centipeda minima and its anti-inflammatory activities / 中草药

Objective: To study triterpenes and their anti-inflammatory activity from the ethanol extract of Centipeda minima. Methods: The compounds were isolated and purified by silica gel, Sephadex LH-20, MCI, ODS and RP-HPLC gel column chromatography, and their structures were elucidated by NMR and MS spectroscopic techniques. The inhibitory activity of the compound on the release of inflammatory mediators NO from mouse macrophages (RAW264.7) induced by lipopolysaccharide (LPS) was determined by Griess method, and then the anti-inflammatory activity of the compounds was evaluated Results: A total of 17 compounds were isolated and identified as: 20-oxo-30-nortaraxastan-3β-yl acetate (1), 3β-acetoxytaraxaster-20-en-30-al (2), 3β-hydroxytaraxaster-20-en-30-al (3), taraxasterol (4), arnidiol (5), 3β,21β-dihydroxy-20(30)-en-taraxastane (6), faradiol (7), pseudotaraxasteryl acetate (8), taraxast-20-ene-3β,30-diol (9), 18α-olean-12-ene-3,11-dione (10), maniladiol (11), 3β- hydroxyolean-12-en-11-one (12), coflodiol (13), lupeol (14), 3β,16β-dihydroxylup-20(29)-ene (15), 16β-hydroxylupa-20(29)- en-3-one (16) and garcinielliptone Q (17). Compounds 2, 5-6, 8-10, 12-13, 15-17 displayed moderate inhibitory activity on theoverproduction of NO in LPS-activated RAW 264.7 mouse macrophage cell lines, IC50 values ranging from 11.9 to 27.1 μmol/L. Conclusion: Compound 1 is a new natural product, and its 1H-NMR and 13C-NMR data was first completely assigned on the basis of 1D and 2D NMR spectroscopic evidence. Compounds 2, 3, 6, 8-10, 12, 13, 15 and 16 are isolated from C. minima for the first time. This work provided theoretical basis for clinical application of C. minima

Quality of life and health of breast cancer woman survivors in Popayan-Colombia / Calidad de vida y salud en mujeres sobrevivientes al cáncer de mama en Popayán- Colombia / Qualidade de vida e saúde em mulheres sobreviventes ao câncer de mama em Popayán, Colômbia

Objective: Identify the quality of life and its association with health parameters for breast cancer women survivors in Popayan, Colombia. Methods: Analytic crosssectional descriptive correlated study, with nonprobability sampling, according to the inclusion criteria of 39 women who survived breast cancer, who received a survey made by the authors which included sociodemographic aspects, evaluation of the quality of life through the questionnaire EORTC QLQ ­ BR23 and measurements such as the Body Mass Index (BMI), blood pressure and blood sugar levels, according to international standards. Results: The health indicators (blood pressure and blood sugar) are found under the normal ranges, while the BMI has a tendency for overweight. Regarding quality of life, high measurements for body image and sexual function were evidenced, while the dimensions of sexual pleasure and future perception showed intermediate measures. Regarding association, moderate correlations were observed between blood pressure and blood sugar levels, with body image and the sexual aspect. Conclusions: An association between the health indicators and the quality of life was observed, demonstrating the need to formulate prevention and intervention actions from an interdisciplinary vision. Also, it was observed that the quality of life requires a review toward self-confidence and relationship with oneself, through workshops or talks that allow to overcome limitations proper of the disease and to move on.

Objetivo: Identificar la calidad de vida y su asociación con parámetros de salud en mujeres sobrevivientes al cáncer de mama en Popayán, Colombia. Métodos: Estudio descriptivo-correlacional de corte transversal analítico, con una muestra no probabilística, de acuerdo con criterios de inclusión de 39 mujeres sobrevivientes al cáncer, a quienes se les aplicó una encuesta de elaboración propia con aspectos socio-demográficos, valoración de la calidad de vida mediante el cuestionario EORTC QLQ - BR23 y mediciones como el Índice de Masa corporal (IMC), presión arterial y niveles de glucemia, según estándares internacionales. Resultados: Los índices de salud (presión arterial y glucemia) se encontraron bajo los rangos de normalidad, mientras que para IMC hay tendencia de sobrepeso. Respecto a la calidad de vida, se evidenciaron mediciones elevadas para la imagen corporal y la función sexual, mientras que las dimensiones de disfrute sexual y percepción a futuro presentaron mediciones intermedias. Respecto a la asociación, se observaron correlaciones moderadas entre la presión arterial y los niveles de glucemia, con la imagen corporal y la parte sexual. Conclusiones: Se evidenció una asociación entre los indicadores de salud y calidad de vida, demostrando la necesidad de plantear acciones de prevención e intervención desde una visión interdisciplinaria. De la misma manera, se observó que la calidad de vida requiere una revisión hacia la autoconfianza y relación consigo misma, a través de talleres o charlas que permitan superar las limitaciones propias de la enfermedad y salir adelante.

Objetivo: Identificar a qualidade de vida e a sua associação com parâmetro de saúde em mulheres sobreviventes ao câncer de mama em Popayán, Colômbia. Métodos: Estudo descritivo-correlacional de corte transversal analítico, estudando uma a mostra probabilística de 39 mulheres sobreviventes ao câncer de mama, que responderam um instrumento de formulação própria com aspectos sociais e demográficos, avaliação da qualidade de vida empregando o questionário EORTC QLQ - BR23 e foram estabelecidos o índice de massa corporal (IMC), a pressão arterial e níveis de glicemia segundo padrões internacionais. Resultados: Os níveis de pressão arterial e glicemia encontraram-se nos rangos de normalidade, entretanto, o IMC mostrou a tendência ao sobrepeso. Na aferição da qualidade de vida, observaram-se medições elevadas na imagem corporal e função sexual, enquanto que as dimensões desfrute sexual e percepção do futuro presentaram aferições intermediarias. Na associação de variáveis houve uma moderada relação entre a pressão arterial e os níveis de glicemia com a imagem corporal e a parte sexual. Conclusões: Evidenciou-se a associação entre os indicadores de saúde e a qualidade de vida, demostrando a necessidade de formular ações de prevenção e intervenção desde uma perspectiva interdisciplinar. Do mesmo modo, observou-se que a qualidade de vida requer uma revisão dirigida à autoconfiança e relação com se mesma, através de oficinas ou conferencias que permitam superar as limitações próprias da doença e seguir em frente.

Study on the Effects of Costunolide on the Proliferation ，Migration and Apoptosis of Human Breast Cancer SK- BR-3 Cells and Its Mechanism / 中国药房

OBJECTIVE：To study the effects of costunolide on the proliferation ，migration and apoptosis of breast cancer SK-BR-3 cells and its mechanism. METHODS ：SK-BR-3 cells in logarithmic growth period were collected and cultured with different concentrations （10，20，30，40，50 μmol/L）of costunolide for 24，48，72 h. Inhibitory rate of costunolide on cell proliferation was detected with CCK- 8. The cells were divided into blank control group and costunolide group （10，20，30 μmol/L）. Hoechst 33258 fluorescence was used to observe the morphology and apoptosis of cells ，and apoptotic rate of cells were calculated. Cell scratch test was used to detect the migration ability of cells and calculate the migration rate. Western blotting was used to detect the relative expression level of Bcl- 2，Bax，Caspase-3 and Cleaved Caspase- 3 in cells. RESULTS ：The proliferation of SK-BR-3 cells were significantly inhibited by costunolide （P＜0.05 or P＜0.01），and it shows a trend of concentration and time dependence. In the blank control group ，cells possessed clear contour ，regular shape and good adherence . Compared with blank control group，the number of cells were decreased significantly in 10，20，30 μmol/L costunolide groups，the cell structure was loose，the volume was reduced ，and the gap became larger ，and most of the cell contour disappeared and became round ，the cell adherence was poor ；cell migration rate and Bcl- 2 protein relative expression level were decreased significantly ，while apoptosis rate and the relative expression level of Bax ，Caspase-3 and Cleaved Caspase- 3 protein were significantly increased （P＜0.05 or P＜0.01）. CONCLUSIONS ： Costunolide can inhibit the proliferation and migration ，and induce apoptosis of human breast cancer SK-BR- 3 cells，mechanism of which may be through up-regulating the expression of Bax ，Caspase-3 and Cleaved Caspase- 3 while down-regulating the expression ofBcl-2.

Cut-off points of the Portuguese version of the Montreal Cognitive Assessment for cognitive evaluation in Parkinson's disease / Valores de corte da versão em português do "montreal cognitive assessment" para avaliação cognitiva na doença de parkinson

ABSTRACT. The Movement Disorder Society has published some recommendations for dementia diagnosis in Parkinson disease (PD), proposing the Montreal Cognitive Assessment (MOCA) as a cognitive screening tool in these patients. However, few studies have been conducted assessing the Portuguese version of this test in Brazil (MOCA-BR). Objective: the aim of the present study was to define the cut-off points of the MOCA-BR scale for diagnosing Mild Cognitive Impairment (PD-MCI) and Dementia (PD-D) in patients with PD. Methods: this was a cross-sectional, analytic field study based on a quantitative approach. Patients were selected after a consecutive assessment by a neurologist, after an extensive cognitive evaluation, and were classified as having normal cognition (PD-N), PD-MCI or PD-D. The MOCA-BR was then applied and 89 patients selected. Results: on the cognitive assessment, 30.3% were PD-N, 41.6% PD-MCI and 28.1% PD-D. The cut-off score on the MOCA-Br to distinguish PD-N from PD-D was 22.50 (95% CI 0.748-0.943) for sensitivity of 85.5% and specificity of 71.1%. The cut-off for distinguishing PD-D from MCI was 17.50 (95% CI 0.758-0.951) for sensitivity of 81.6% and specificity of 76%.

RESUMO. A Movement Disorder Society publicou algumas recomendações para o diagnóstico de demência na doença de Parkinson (DP), propondo o Montreal Cognitive Assessment (MOCA) como ferramenta de triagem cognitiva nesses pacientes. Entretanto, poucos estudos foram aplicados à versão em português (MOCA-BR). Objetivo: o presente estudo tem o objetivo de definir os valores de corte na escala de MOCA-BR para diagnosticar o Comprometimento Cognitivo Leve (DP-CCL) e Demência (DP-D) em pacientes com DP. Métodos: trata-se de um estudo transversal, analítico, com uma abordagem quantitativa. Os pacientes foram selecionados depois de avaliações consecutivas por um neurologista, após avaliação cognitiva extensa, e foram classificados como cognição normal (DP-N), DP-CCL e DP-D e então o MOCA-BR foi aplicado, sendo selecionados 89 pacientes. Resultados: na avaliação cognitiva, foram encontrados 30.3% de DP-N, 41.6% de DP-CCL e 28.1% DP-D. O valor de corte no MOCA-BR para distinguir entre DP-N de DP-D foi 22.5 (IC 95%; 0.748-0.943), sensibilidade de 85.5% e especificidade de 71.1%. Para distinguir DP-P de CCL, o ponto de corte foi de 17.5 (IC 95%; 0.758-0.951), sensibilidade de 81.6% e especificidade de 76%.

Chemical constituents from Calystegia sepium / 中草药

Objective To study the chemical constituents from Calystegia sepium. Methods The compounds were isolated and purified by silica gel column chromatography, Sephadex LH-20, ODS column chromatography, preparative HPLC and so on. Their structures were determined on the basis of physicochemical properties and their spectroscopic data, as well as literatures. Results A total of 20 compounds were separated and identified as tyrosol (1), quercetin-3-O-β-D-glucopyranoside (2), scutellarein-7-O-β- glucopyranoside (3), kaempferol-3-O-β-D-glucopyranoside (4), kaempferol-3-O-galactoside (5), kaempferol-3-O-β-robinobioside (6), nicotiflorin (7), rutin (8), uridine (9), tachioside (10), 2-methoxy-4-(2’-hydroxyethyl)-phenyl-1-O-β-D-glucopyranoside (11), 4-hydroxyphenethylol-4-O-β-D-glucopyranoside (12), polybotrin (13), pyridine-3,5-dicarboamide (14), skimmin (15), benzylalcohol-β-D-glucopyranoside (16), 5-hydroxyl-2-hydroxymethylpyridine (17), picein (18), 5-hydroxymethyl-furaldehyde (19), and sessiline (20). Conclusion Compounds 1-20 are isolated from Calystegia sepium for the first time.

Effect of tamoxifen on invasiveness of breast cancer SK-BR-3 cells and its mechanism / 中华内分泌外科杂志

Objective To investigate the effect of tamoxifen (TAM) on the invasiveness of breast cancer SK-BR-3 cells and its mechanism.Methods The SK-BR-3 breast cancer cells in logarithmic growth period were cultured for 24 h by theserum-free RPMI 1640 culture medium,and then were divided into control group (serumfree RPMI 1640 medium) and TAM group(serum-freeRPMI 16400.5,2.5,12.5 mol·L-1TAM).Western blot was used to detect matrix metalloproteinase-9 (MMP-9),matrix metalloproteinase-2 (MMP-2),and vascular endothelial growth factor (VEGF) in groups.Scratch and transwelhests were used to detect the invasiveness of SK-BR-3 cells.Results The results of scratch test and Transwell experiment showed that the relative invasive ability of SKM-BR-3 cells in TAM (0.5,2.5,12.5 mol/L) group was significantly higher than that in the control group,and the difference was statistically significant (P＜0.05).The invasive ability of SK-BR-3 cells increased with the increase of TAM con centration.The results of Western blot showed that the expression levels of VEGF,MMP-9 and MMP-2 in SK-BR-3 cells of TAM group were average.It was significantly higher than the control group,and the expression levels of VEGF,MMP-9 and MMP-2 in SK-BR-3 cells increased gradually with the increase of TAM concentration.Conclusion Tamoxifen may play the role of estrogens and promote the invasiveness of SK-BR-3 breast cancer cells,via increasing the expression levels of VEGF,MMP-9 and MMP-2.

Solidão e Depressão: Relações com Características Pessoais e Hábitos de Vida em Universitários / Loneliness and Depression: Relations with Personal Characteristics and Life Habits in University Students

RESUMO Este trabalho avaliou a solidão e a depressão em universitários, verificando sua relação com hábitos, características do curso e suporte social. Participaram 574 universitários. Utilizou-se a Escala de Solidão UCLA-BR, Escala de Suporte Social, Questionário sobre a Saúde do Paciente e questionário complementar. Houve triagem para depressão em 38,7% dos estudantes e 53% sentia solidão mínima. A maioria dos estudantes fazia quatro ou mais refeições, dormia entre 6 e 8 horas, considerava ter amigos e lazer. Não fumavam, mas bebiam. Houve correlação entre solidão e depressão (rho = 0,42) e dessas condições com hábitos dos universitários, área do curso e suporte social. Conclui-se que o estado emocional de universitários avaliados é preocupante e intervenções são necessárias.

ABSTRACT This study evaluated loneliness and depression of college students to verify its relationship with their habits, course characteristics, and social support. It was used the UCLA-BR Loneliness Scale, Social Support Scale, Patient Health Questionnaire, and a complementary questionnaire to evaluate 574 students. Depression was screened in 38.7% of the students, and 53% of them showed minimal loneliness. Most students had four or more daily meals, slept 6 to 8 hours, considered having friends and leisure, did not smoke, but usually drank. There was a correlation between loneliness and depression (rho = 0.42) and of these with the students' habits, course area, and social support. It was concluded that the emotional state of the students is worrying and that interventions are necessary.

Associação entre o Functional Movement Screen e aptidão física em escolares / Association between Functional Movement Screen and physical fitness among schoolchildren

É evidente que o comportamento sedentário esta relacionado com a ocorrência de diversas doenças e aumento do risco de morte. Especificamente na população jovem, estudos mostram que os níveis de atividade física e a aptidão física de crianças e adolescentes são cada vez menores. Informações sobre o desempenho e a qualidade de ações motoras de crianças e adolescentes são necessárias para a elaboração de programas de educação física, de esporte e de atividades físicas em geral. Para tanto, existem diferentes ferramentas de avaliação que visam observar as características do comportamento motor, entre elas o Functional Movement Screen e a bateria de testes do Projeto Esporte Brasil. Este estudo buscou verificar a associação entre o escore do Functional Movement Screen e as variáveis de aptidão física da bateria de testes do Projeto Esporte Brasil em escolares. Participaram do estudo 24 escolares (15 meninos) de 12 anos de idade. Os dados foram descritos utilizando-se média, desvio padrão (variáveis com distribuição normal), mediana e diferença interquartis (variáveis sem distribuição normal). Para a associação entre o escore do Functional Movement Screen e as variáveis de aptidão física foram utilizados os coeficientes de correlação de Pearson (variáveis com distribuição normal) e Spearman (variáveis sem distribuição normal). Foram encontradas associações moderadas e significativas entre o Functional Movement Screen e o teste de flexibilidade (r= 0,69 p=0,004), força explosiva de membros superiores (r=0,679 p=0,005) e teste de velocidade de 20 metros (r= -0,57 p=0,025) apenas entre os meninos. Evidencia-se com estes dados uma capacidade limitada do Functional Movement Screen estar relacionado com a aptidão física de escolares...(AU)

It is evident that sedentary behavior is related to the occurrence of several diseases and increased risk of death. Specifically in the young population, studies show that the levels of physical activity and physical fitness of children and adolescents are decreasing. Information about the performance and quality of the motor actions in children and adolescents are necessary to physical education, sports and general physical activities programs elaboration. Therefore, there are different tools of evaluation that aim to observing the motor behavior characteristic, among then, the Function Movement Screen and the Projeto Esporte Brasil battery of tests. This study aimed to verifying the association between Functional Movement Screen score and the physical fitness variables of the Projeto Esporte Brasil battery of tests in schoolchildren. Participants were 24 schoolchildren (15 boys) of 12 years old. Data were described using mean, standard deviation (parametric variables), median and interquartile difference (non-parametric variables). For the association between Functional Movement Screen score and physical fitness variables were used Pearson's coefficient correlations (parametric variables) and Sperman's coefficient correlation (non-parametric variables). Significant and moderate associations were found between Functional Movement Screen score and flexibility (r=.69; p=.004), lower limb power (r=.68; =.005) and sprint test (r=-.57; p=.025) only in boys. These results evidence a limited capacity of the Functional Movement Screen to be associated to physical fitness in schoolchildren...(AU)

Production and quality of silages pearl millet and paiaguas palisadegrass in monocropping and intercropping in different forage systems / Produção e qualidade da silagem de milheto e capim-paiaguás em monocultuvo e consorciado em diferentes sistemas forrageiros

With the arrival of new early genotypes and high nutritive value and productive potential, millet has been gaining prominence in recent years for the production of silage, and it promises lower costs of production. Thus, the consortium of millet with tropical forages can increase the production of silage, providing more food to be used in the offseason, where there is low availability of forage. The objective of this study was to evaluate the dry mass production, fermentative characteristics and chemical-bromatological of pearl millet silage and Paiaguas palisadegrass in monocropping and intercropping in different forage systems in the second cropping season. The experimental design consisted of randomized blocks with four replicates. The treatments consisted of silage of the following forage systems: monocropped pearl millet, monocropped Paiaguas palisadegrass, pearl millet intercropped in rows with Paiaguas palisadegrass, pearl millet intercropped inter-row with Paiaguas palisadegrass, and pearl millet oversown and intercropped with Paiaguas palisadegrass (totaling 20 experimental plots). The consortium of pearl millet with Paiaguas palisadegrass contributed to raise the dry matter contents of the silages, except in the system using the overgrowth. Pearl millet silages monocropped and intercropped with Paiaguas palisadegrass presented reductions in pH and ammoniacal nitrogen values as well as favor lactic fermentation. Pearl millet monocropped and intercropped silage exhibited better fermentative and bromatological characteristics than silage obtained from monocropped Paiaguas palisadegrass. Therefore, creating silage from intercropped forages provides an interesting supplemental roughage option that can be used during the offseason for animal feeding.

Com a chegada de novos genótipos precoces e de alto valor nutritivo e potencial produtivo, o milheto vem ganhando destaque nos últimos anos para a produção de silagens, com menor custo de produção. Com isso, o consórcio do milheto com forrageiras tropicais, pode aumentar a produção da silagem, proporcionando maior quantidade de alimento para ser utilizado na entressafra, onde se tem baixa disponibilidade de forragem. Sendo assim, objetivou-se avaliar a produção de massa seca, características fermentativas e a composição químico-bromatológica da silagem de milheto e capim-paiaguás em monocultivo e consorciado em diferentes sistemas forrageiros, na safrinha. O delineamento experimental utilizado foi em blocos casualizados, com quatro repetições. Os tratamentos foram constituídos da silagem dos seguintes sistemas forrageiros: milheto em monocultivo, capim-paiaguás em monocultivo, milheto consorciado com capim-paiaguás na linha, milheto consorciado com capim-paiaguás na entrelinha e milheto consorciado com capimpaiaguás na sobressemeadura, totalizando 20 silos experimentais. O consórcio do milheto com capim-paiaguás contribuiu para elevar os teores de matéria seca das silagens, exceto, no sistema utilizando a sobressemeadura. As silagens de milheto em monocultivo e consorciadas com capim-paiaguás apresentaram reduções nos valores de pH e nitrogênio amoniacal, bem como, favorecem a fermentação lática. As silagens de milheto em monocultivo e consorciadas apresentaram melhores características fermentativas e bromatológicas quando comparadas com silagem de capim-paiaguás em monocultivo. Portanto, silagens produzidas nos sistemas consorciados constitui interessante opção de volumosos suplementar para alimentação dos animais no período de entressafra.

Clinical significance of combined detection of serum C3bR and IL-6 in patients with traumatic brain injury / 国际检验医学杂志

Objective To investigate clinical significance of combined detection of serum C 3bR and IL-6 in patients with traumatic brain injury.Methods 48 patients with TBI admitted in 371 Central Hospital of PLA from May 2016 to May 2017 were randomly selected as the subjects,including 26 males and 22 females,aged 25 to 60 years,with an average age of(43.7 ± 5.8)years.Admission according to the injury by Glasgow coma score(GCS):≤7 points in 18 cases,≥8 points in 30 cases.Another 30 healthy people who were admitted to the hospital for the same period were selected as the control group.Serum C3bR and IL-6 levels were meas-ured by ELISA in the study group at 1,3 and 7 days after admission,and the expression of C3bR and IL-6 were analyzed.Results The level of serum C3bR in the first and the third day after traumatic brain injury was significantly lower than that in the control group,and the level of IL-6 after craniocerebral injury was signifi-cantly higher than that of the control group(P<0.05).In the observation group,the level of C3bR was signif-icantly higher than that of ≤ 7,and the level of IL-6 was significantly lower than that of the severe GCS score ≥8(P<0.05).In this study,the serum C3bR,IL-6 and the two joint tests were 0.624,0.641 and 0. 900,respectively area.Conclusion Serum C3bR and IL-6 combined detection can significantly improve the sensitivity of traumatic brain injury diagnosis,so as to understand the severity of traumatic brain injury,w hich could help to assess the disease and determine the prognosis,and have important clinical value.

Determination of seven effective constituents in crude and processed Cuscuta australis by quantitative analysis of multi-components with single marker / 中草药

Objective To establish a quantitative analysis of multi-components with single marker (QAMS) for the simultaneous determination of chlorogenic acid, cryptochlorogenin acid, caffeic acid, hyperoside, isoquercitrin, astragalin, kaempferol in crude and processed Cuscuta australis, which is proved to be a scientific and feasible method in the quality analysis in C. australis. Methods Six relative correction factors (RCFs) of chlorogenic acid, cryptochlorogenin acid, caffeic acid, isoquercitrin, astragalin, kaempferol was established in the HPLC method with the hyperoside as the internal standard (IS), which was to calculate the mass fraction of each. The mass fraction of seven effective constituents in crude and processed C. australis was calculated by the external standard method (ESM) at the same time. Compared with the content results determined by the ESM and QAMS, the feasibility and accuracy of QAMS method were verified. Results The relative correction factor (RCF) was perfect. The detection calculated by QAMS was consistent with the results by ESM. Conclusion The method with a single marker, using the hyperoside as IS, is accurate and feasible for the quantitative analysis of six other effective constituents in C. australis.

Cloning and analysis of rDNA ITS sequences from 11 medical plants in Calanthe / 中草药

Objective: To provide evidences for molecular identification, sequence comparisons of 11 rDNA ITS in Calanthe plants were performed. Methods: ITS sequences of Calanthe plants were isolated by using Polymerase Chain Reaction (PCR) method, and then analyzed by software namely Clustal, GenDoc, and Mega. Results: The full ITS sequences of 11 Calanthe plants ranged from 652 to 661 bp; ITS1 and ITS2 sequences were 228-230 bp and 256-264 bp in length, respectively, while their 5.8 S sequences were identical with a length of 167 bp. The variable sites in both ITS1 and ITS2 were 44 and 46, respectively. Parsimony information sites of ITS1 and ITS2 were 22 and 24, however, there was no variable and information sites indentified in 5.8 S sequences. Genetic distance among 11 Calanthe species varied from 0.002 to 0.081, and they were divided into three groups in a phylogenetic tree with species numbers of 5, 4, and 2 in I-III groups, respectively. Conclusion: The ITS sequences of 11 Calanthe plants were rich in information sites, which could be applied for molecular identification.

Fingerprint profiling of Macleaya cordata fruits from different regions / 中草药

Objective To establish the fingerprint profiling of fruits of Macleaya cordata and study the method for its quality evaluation. Methods The fingerprint profiling of M. cordata fruits from different regions was established using high performance liquid chromatography (HPLC) based on the local standard which was the determination method of the content of protopine, allocryptopine, sanguinarine, and chelerythrine from Changsha of Hunan Province in 2009. The principal component analysis (PCA) and cluster analysis (CA) were performed to explore the correlation among the common fingerprint peaks, origins, and quality of M. cordata fruits. Results Eleven common fingerprint peaks were identified in the fingerprint profiling of chemical constituents of M. cordata fruits from different regions. M. cordata fruits produced from eight areas were classified into two classes by PCA and CA method, and there were five common peaks, including peak 5, 7, 8, 9, and 11 with significant contribution on the regional difference of the fingerprint. Also, common peak 6 was the right peak as the reference peak because of its less variation, appropriate retention time and intensity. Conclusion The fingerprint profiling of chemical constituents of M. cordata fruits established in this study has good precision, repeatability, and stability, which can be used to evaluate the quality of fruits of M. cordata from different producing areas.